WebPCDNA3-FlipGFP (Casp3 cleavage seq) T2A mCherry was a gift from Xiaokun Shu (Addgene plasmid # 124428 ; http://n2t.net/addgene:124428 ; RRID:Addgene_124428) For your References section: Designing a green fluorogenic protease reporter by flipping a beta strand of GFP for imaging apoptosis in animals. WebGeneral Tools: Plasmids expressing Cas12a/Cas13a, Reverse Transcriptases, etc. that may be useful for detection assays and more. Research Articles: A list of free access articles on COVID-19 research. Protocols: Protocols that may be useful for COVID-19 research. ... FlipGFP-based activity reporter of SARS-CoV-2 main protease Mpro. (Unpublished)
IJMS Free Full-Text Genetically Encoded Fluorescent Sensors for ...
WebJan 31, 2024 · As demonstrated in the Flip-GFP system, we connected two small β strands, nLuc (β9) and nLuc (β10), with designed heterodimerizing coil linkers, E5 and K5, … WebApr 13, 2024 · Next, we extended the study of reporter assay (Supplementary Fig. 1) ... (Supplementary Fig. 2), in which an inactive flipGFP could be activated by a specific cleavage of NSP5 recognition site. philip oldham
Discovery of SARS-CoV-2 papain-like protease inhibitors ... - bioRxiv
WebSemantic Scholar profile for Maura V Gongora, with 6 highly influential citations and 5 scientific research papers. WebJan 1, 2024 · The FlipGFP assay was also applied to validate the structurally disparate M pro inhibitors reported in the literature. Lastly, we introduce recent progress in identifying … FlipGFP is a novel fluorogenic reporter that has a large dynamic range and high brightness. This reporter achieves approximately 100-fold increase in fluorescence after cleavage by executioner caspases. Failure for cells to die or cells dying at the wrong time and/or place can lead to an array of diseases and … See more GFP contains 11 β-strands and one central ɑ-helix that can be split into 3 parts: (1) β-strands 1-9 (β1-9) and the one ɑ-helix, (2) β-strand 10 (β10), and (3) β-strand 11 (β11). Each part contains components and … See more To add to the fluorogenic reporter toolkit, the Shu Lab also designed a red fluorogenic protease reporter based on superfolder Cherry … See more The Shu Lab successfully used FlipGFP to study apoptosis in HeLa cells, zebrafish embryos, and Drosophila. They treated HeLa cells expressing FlipGFP with staurosporine, which … See more philip oldroyd