Optical tweezers indentation cell
WebDec 31, 2024 · Cortical stiffness of keratinocytes measured by lateral indentation with optical tweezers Keratin intermediate filaments are the principal structural element of epithelial cells. Their importance in providing bulk cellular stiffness is well recognized, but their role in the mechanics of cell cortex is less understood. WebMar 4, 2024 · More information: Xuchen Shan et al. Optical tweezers beyond refractive index mismatch using highly doped upconversion nanoparticles, Nature Nanotechnology (2024).DOI: 10.1038/s41565-021-00852-0
Optical tweezers indentation cell
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WebJan 1, 2016 · Cell elasticity can be locally measured by pulling membrane tethers, stretching or indenting the cell using optical tweezers. In this paper, we propose a simple approach to perform cell indentation at pN forces by axially moving the cell against a trapped microbead. The elastic modulus is calculated using the Hertz-model. WebUsing conventional optical tweezers as actuators for micromachines is likely to speed the adoption of lab-on-a-chip and related technologies for medical diagnostics, environmental …
WebWe investigated the elastic modulus of HBL-100 and MDA-MB-231 cell lines considering the optical tweezers indentation experiment described in section 2. In order to measure their elasticity, the cells were indented in the vertical direction by a 3 µm diameter silica bead that was held in the optical trap. WebNov 1, 2003 · Development of experimental techniques based on optical or laser traps (also commonly referred to as optical or laser tweezers) has facilitated mechanical deformation of whole biological cells at forces ranging from tens to hundreds of pN.
http://softmatterlab.org/2024/04/11/j-phys-photonics-5-022501-2024/ WebInvestigation into local cell mechanics by atomic force microscopy mapping and optical tweezer vertical indentation G Coceano1,2, M S Yousafzai1,2,3,WMa4, F Ndoye5,6, L Venturelli1,2,3, I Hussain4, S Bonin7, J Niemela5, G Scoles3, D Cojoc1 and E Ferrari4 1CNR-IOM National Research Council—Institute of Materials, Area Science Park, Basovizza, S.S. …
WebNov 29, 2024 · The rigorous understanding of DNA elasticity that emerged from early studies set the stage for optical tweezers to begin probing the translocases which operate on DNA.
WebDec 18, 2015 · Here we combine two complementary techniques, atomic force microscopy (AFM) and optical tweezer microscopy (OTM), providing a comprehensive mechanical … simpson mccleary washingtonWebcell stiffness; optical tweezers; vertical indentation 1. Introduction In the last two decades, cell mechanics has been acknowledged as a possible biomarker for numerous diseases, most importantly cancer[1,2]. The mechanical properties of a cell play a pivotal role in many cellular processes like cell motility, adhesion, cell division and ... simpson mccreath trustWebWe report on the modification of mechanical properties of breast cancer cells when they get in contact with other neighboring cells of the same type. Optical tweezers vertical indentation was employed to investigate cell mechanics in isolated and contact conditions, by setting up stiffness as a marker. Two human breast cancer cell lines with different … simpson maxidry 505srWeb15 hours ago · Here, we propose a completely non-invasive method for cell manipulation by combining a microcapillary microfluidic device and an optical fiber tweezer. We demonstrate that Chlorella cells inside the microcapillary channel can be successfully trapped and manipulated by an optical fiber probe located outside of the microcapillary, … simpson mcmahan glick and burfordWebMar 1, 2015 · However, instead of utilizing a movable bead and a single optical trap, the effectiveness of indentation assays in optically trapped a bead was examined when … simpson mazzoli act of 1986Web摘要： The peculiarity of red blood cell interaction was studied by optical tweezers. The intercellular interaction force in cell pairs and the role of cell deformation and adhesion time in interaction dynamics were evaluated and discussed. simpson mclearnon \u0026 ferguson ltdWebApr 12, 2024 · In a fiber-based optical tweezer system, it is a common practice to insert the fiber probe into the sample solution to perform the tweezer function. Such a configuration of the fiber probe may lead to unwanted contamination and/or damage to the sample system and is thus potentially invasive. Here, we propose a completely non-invasive method for … razerstore mouse